CryoEM Biophysical approach Hedgehog signal Escherichia coli Blood proteins HasR DDM Hedgehog signal intrinsically disordered protein small-angle X-ray scattering Membrane protein reconstitution Phospholipid Haute pression HP Flow cytometry Gramicidin Hydration Expression Bacteriocin ABC ATP-binding cassette Amphipol Cerebrovascular function Chemotherapy resistance drug efflux Chlamydia muridarum Endonuclease Amphipols Membrane proteins Arabinogalactan Surfactants Bacteria CryoEM of membranes fused by NS4B peptide Fluorescence Colicin Dimyristoylphosphatidylcholine Lipids Bacillus subtilis ATPase activity Chemical biology Corynebacterium glutamicum Detergents Mycobacterium Adenosine receptor A 2A biophysical approach NMR mass spectrometry molecular pharmacology expression purification reconstitution ligands techniques review Bilayer Diffusion Solid-state NMR Cell envelope E coli Electron transfer Fluoroquinolone GPCR Adenosine receptor A 2A Cell-wall Membrane protein Amphipol-stabilized integral membrane protein FRAP Electron microscopy Density map Circular Dichroism G glycoprotein from rabies virus High pressure HP Haem DNA-binding protein Corynebacteriales Glycosylation DPC Heme Concussion NMR Dynamic filters In vitro synthesis FLIM Biochemistry SMA co-polymers Ice thickness Ab initio modeling DNA gyrase Chemistry G protein-coupled receptors Human Cell markers Fourier Transform Infrared F1Fo-ATPase Gram-positive and Gram-negative bacteria Intrinsically disordered protein Cardiolipin Cryo-EM of membranes disordered by NS4B Air-water interface A8-35 formulation study Small-angle X-ray scattering Bilayers Bacterial nutrient transporter Hème ci Phospholipids Endothelial cells HCV-NS4B induced membrane disorder Detergent Action mechanism BBB Hedgehogh signal Analytical modeling Cryo-electron microscopy A8 35 Extraction