The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei - Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé - UMS 37 PASS Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2020

The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei

Résumé

Asexual blood stages of the malaria parasite are readily amenable to genetic modification via homologous recombination, allowing functional studies of parasite genes that are not essential in this part of the life cycle. However, conventional reverse genetics cannot be applied for the functional analysis of genes that are essential during asexual blood-stage replication. Various strategies have been developed for conditional mutagenesis of Plasmo-dium, including recombinase-based gene deletion, regulatable promoters, and mRNA or protein destabilization systems. Among these, the dimerisable Cre (DiCre) recombinase system has emerged as a powerful approach for conditional gene deletion in P. falciparum. In this system, the bacteriophage Cre is expressed in the form of two separate, enzymati-cally inactive polypeptides, each fused to a different rapamycin-binding protein. Rapamycin-induced heterodimerization of the two components restores recombinase activity. We have implemented the DiCre system in the rodent malaria parasite P. berghei, and show that rapamycin-induced excision of floxed DNA sequences can be achieved with very high efficiency in both mammalian and mosquito parasite stages. This tool can be used to investigate the function of essential genes not only in asexual blood stages, but also in other parts of the malaria parasite life cycle.

Domaines

Parasitologie
Fichier principal
Vignette du fichier
journal.pone.0236616.pdf (2.69 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-02996556 , version 1 (09-11-2020)

Identifiants

Citer

Priyanka Fernandes, Sylvie Briquet, Delphine Patarot, Manon Loubens, Bénédicte Hoareau, et al.. The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei. PLoS ONE, 2020, 15 (10), pp.e0236616. ⟨10.1371/journal.pone.0236616⟩. ⟨hal-02996556⟩
177 Consultations
55 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More