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, Higher magnification images taken from the boxed areas in the OE and RE are shown. BGs (arrow) are filled with glycoconjugated materials that are AB-PAS-positive and specifically recognized by the anti-GFP antibody, but not the anti-Muc5ac antibody. Mucous cells of the RE expressed Muc5ac

, Immunohistochemical analysis of Muc5b and Muc5ac in nose coronal sections of the proximal (T1) nasal airway of WT mice. Serial sections were stained with antibodies against Muc5b (green) and Muc5ac (red)

, Immunohistochemical analysis of Muc5b and Muc5ac in nose coronal sections of the proximal (T3) nasal airway of WT mice. Serial sections were stained with antibodies against Muc5b and Muc5ac. Higher magnification images of the OE and RE are shown. Muc5b staining was observed in BGs (arrows) and BGs ducts in the OE. Goblet cells of the RE produced both Muc5b and Muc5ac

, Multilabel immunohistochemical analysis of Muc5b with MAA or UEA-1 lectin in the OE and RE of coronal section T3 of the nasal airway from Muc5b -/-mice and with MAA and UEA-1 lectins in the OE and RE of coronal section T2 of the nasal airway from Muc5b-GFP reporter and WT mice. a. BGs underlying the OE are not visible (depleted) and are not stained with either the Muc5b antibody or MAA. Mucous cells of the RE were stained by UEA-1 and very faintly by MAA. Lu: lumen. Scale bar = 50 ?m. b. High magnification images of the OE and RE are shown. MAA lectin binding was observed in BGs (arrows) underlying the OE and in Bowman's gland ducts (d)