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Protein-mediated dethreading of a biotin-functionalised pseudorotaxane

Abstract : In this article, we describe the synthesis of new biotin-functionalised naphthalene derivatives 3 and 4 and their complexation behaviour with avidin and neutravidin using a range of analytical techniques. We have shown using 2-(4′-hydroxyazobenzene)benzoic acid displacement and ITC experiments, that compounds 3 and 4 have the propensity to form reasonably high-affinity bioconjugates with avidin and neutravidin. We have also demonstrated using 1H NMR, UV-vis and fluorescence spectroscopy that the naphthalene moiety of 3 and 4 facilitates the formation of pseudorotaxane-like structures with 1 in water. We have then investigated the ability of avidin and neutravidin to modulate the complexation between 1 and 3 or 4. UV-vis and fluorescence spectroscopy has shown that in both cases the addition of the protein disrupts complexation between the naphthalene moieties of 3 and 4 with 1.
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https://hal.univ-lille.fr/hal-02544680
Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 16 avril 2020 - 15:04:06
Dernière modification le : samedi 18 avril 2020 - 01:39:04

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Stuart T Caldwell, Catherine Maclean, Mathis Riehle, Alan Cooper, Margaret Nutley, et al.. Protein-mediated dethreading of a biotin-functionalised pseudorotaxane. Organic and Biomolecular Chemistry, 2014, Organic & Biomolecular Chemistry, 12, pp.511-516. ⟨10.1039/c3ob41612g⟩. ⟨hal-02544680⟩

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