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The mirna-targeted transcriptome of porcine alveolar macrophages upon infection with porcine reproductive and respiratory syndrome virus

Abstract : Host miRNAs are known to modulate the cell response to virus infections. We characterized the miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain of PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). We performed the immunoprecipitation of RISC (RNA-induced Silencing Complex) followed by microarray analysis of the RISC-bound miRNA targets (RIP-Chip) to evaluate the relative enrichment or depletion of expressed genes in RISC. The miRNA-mediated regulation occurred early after PRRSV infection and decreased fast (1,241 and 141 RISC-bound genes at 7 h and 10 h post-infection, respectively); it affected several cell functions with evidence of miRNA buffering of upregulated interferon-related genes. Eight miRNAs were highly enriched in RISC of both control and infected cells with no evidence of differential expression. Although miR-335-5p was the miRNA with most predicted targets among enriched RISC-bound genes, no effects on surface markers, cytokine expression and PRRSV replication were detected upon miR-335-5p mimics of primary PAMs. Our results do not point to specific miRNA-driven mechanisms regulating the early response to infection with this PRRSV 1.1 strain and indicate that the miRNome expressed by steady-state PAMs reacts promptly to counterbalance PRRSV infection by a pervasive modulation of host functions.
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https://hal.univ-lille.fr/hal-02573144
Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 14 mai 2020 - 10:54:46
Dernière modification le : samedi 1 août 2020 - 12:02:02

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Sophie Dhorne-Pollet, Elisa Crisci, Nuria Mach, Patricia Renson, Florence Jaffrezic, et al.. The mirna-targeted transcriptome of porcine alveolar macrophages upon infection with porcine reproductive and respiratory syndrome virus. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.3160. ⟨10.1038/s41598-019-39220-3⟩. ⟨hal-02573144⟩

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