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Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Abstract : The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.
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https://hal.univ-lille.fr/hal-03022120
Contributeur : Lilloa Université de Lille <>
Soumis le : mardi 24 novembre 2020 - 16:24:26
Dernière modification le : mercredi 25 novembre 2020 - 03:29:16
Archivage à long terme le : : jeudi 25 février 2021 - 20:40:08

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Armance Gelaude, Sylvain Slaby, Katia Cailliau-Maggio, Matthieu Marin, Arlette Lescuyer, et al.. Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes. Cells, MDPI, 2020, Cells, 9 (1), pp.237. ⟨10.3390/cells9010237⟩. ⟨hal-03022120⟩

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