Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis

Abstract : Lignin is present in plant secondary cell walls and is among the most abundant biological polymers on Earth. In this work we investigated the potential role of the UGT72E gene family in regulating lignification in Arabidopsis. Chemical determination of floral stem lignin contents in ugt72e1, ugt72e2, and ugt72e3 mutants revealed no significant differences compared to WT plants. In contrast, the use of a novel safranin O ratiometric imaging technique indicated a significant increase in the cell wall lignin content of both interfascicular fibers and xylem from young regions of ugt72e3 mutant floral stems. These results were globally confirmed in interfascicular fibers by Raman microspectroscopy. Subsequent investigation using a bioorthogonal triple labelling strategy suggested that the augmentation in lignification was associated with an increased capacity of mutant cell walls to incorporate H-, G-, and S-monolignol reporters. Expression analysis showed that this increase was associated with an up-regulation of LAC17 and PRX71, which play a key role in lignin polymerization. Altogether, these results suggest that UGT72E3 can influence the kinetics of lignin deposition by regulating monolignol flow to the cell wall as well as the potential of this compartment to incorporate monomers into the growing lignin polymer.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-lille.fr/hal-03030963
Contributeur : Lilloa Université de Lille <>
Soumis le : lundi 30 novembre 2020 - 12:11:10
Dernière modification le : vendredi 26 mars 2021 - 10:20:02
Archivage à long terme le : : lundi 1 mars 2021 - 18:52:27

Fichier

P20.17 ijms-21-06094-1.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Fabien Baldacci-Cresp, Julien Le Roy, Brigitte Huss, Cedric Lion, Anne Creach, et al.. UDP-GLYCOSYLTRANSFERASE 72E3 Plays a Role in Lignification of Secondary Cell Walls in Arabidopsis. International Journal of Molecular Sciences, MDPI, 2020, International journal of molecular sciences, 21 (17), pp.6094. ⟨10.3390/ijms21176094⟩. ⟨hal-03030963⟩

Partager

Métriques

Consultations de la notice

19

Téléchargements de fichiers

45