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Analysis of an improved Cyanophora paradoxa genome assembly

Abstract : Glaucophyta are members of the Archaeplastida, the founding group of photosynthetic eukaryotes that also includes red algae (Rhodophyta), green algae, and plants (Viridiplantae). Here we present a high-quality assembly, built using long-read sequences, of the ca. 100 Mb nuclear genome of the model glaucophyte Cyanophora paradoxa. We also conducted a quick-freeze deep-etch electron microscopy (QFDEEM) analysis of C. paradoxa cells to investigate glaucophyte morphology in comparison to other organisms. Using the genome data, we generated a resolved 115-taxon eukaryotic tree of life that includes a well-supported, monophyletic Archaeplastida. Analysis of muroplast peptidoglycan (PG) ultrastructure using QFDEEM shows that PG is most dense at the cleavage-furrow. Analysis of the chlamydial contribution to glaucophytes and other Archaeplastida shows that these foreign sequences likely played a key role in anaerobic glycolysis in primordial algae to alleviate ATP starvation under night-time hypoxia. The robust genome assembly of C. paradoxa significantly advances knowledge about this model species and provides a reference for exploring the panoply of traits associated with the anciently diverged glaucophyte lineage.
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https://hal.univ-lille.fr/hal-03095856
Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 14 janvier 2021 - 15:16:57
Dernière modification le : jeudi 21 janvier 2021 - 03:09:00

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Dana C Price, Ursula W Goodenough, Robyn Roth, Jae-Hyeok Lee, Thamali Kariyawasam, et al.. Analysis of an improved Cyanophora paradoxa genome assembly. DNA Research, Oxford University Press (OUP), 2019, DNA Research, 26 (4), pp.287-299. ⟨10.1093/dnares/dsz009⟩. ⟨hal-03095856⟩

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