Accéder directement au contenu Accéder directement à la navigation
Chapitre d'ouvrage

Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)

Abstract : Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes. In the virus-induced gene silencing (VIGS) method, it is possible to take advantage of this mechanism by inserting a plant nucleic fragment within the viral genome to knock down the corresponding gene. This tool has been used in many species as a fast and easy reverse genetics technique in order to gain information on the role of genes with poorly understood functions. Here we describe in detail two Agrobacterium-mediated infection protocols in flax, based on a whole plant vacuum infiltration and a leaf syringe infiltration that systemically impact the transcript levels in the stem.
Type de document :
Chapitre d'ouvrage
Liste complète des métadonnées

https://hal.univ-lille.fr/hal-03101230
Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 7 janvier 2021 - 08:02:14
Dernière modification le : lundi 8 février 2021 - 16:24:37

Identifiants

Collections

Citation

Maxime Chantreau, Godfrey Neutelings. Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum). Virus-Induced Gene Silencing in Plants Methods and Protocols, Springer US; Vincent Courdavault and Sébastien Besseau / Humana Press, pp.65-74, 2020, ⟨10.1007/978-1-0716-0751-0_6⟩. ⟨hal-03101230⟩

Partager

Métriques

Consultations de la notice

14