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Article Dans Une Revue The International Arabic Journal of Antimicrobial Agents Année : 2018

Identification of the first bacteriocin isolated in Lebanon extracted via a modified adsorption-desorption method and its potential food application

Imad Al Kassaa
Mazen Zaylaa
  • Fonction : Auteur
Samah Hussein
  • Fonction : Auteur
Khaled El Omari
  • Fonction : Auteur
Monzer Hamze
Hoda Yusef
  • Fonction : Auteur
Fouad Dabboussi
  • Fonction : Auteur

Résumé

Introduction: The raw goat milk is considered as a good source of novel bacteriocinogenic lactic acid bacteria (LAB) strains. Method: The bacteriocin, which named enterocin CMUL20-2 was secreted by Enterococcus faecium CMUL20-2. This bacterial strain was originally isolated from raw goat’s milk, was extracted by using a modified adsorption-desorption method and purified via RP-HPLC. antimicrobial activity was tested against several pathogenic and spoilage microbes. Results: The enterocin CMUL20-2 showed a strong adsorption on cell wall of producer strain even in acidic environment which facilitate its extraction in only two simple steps. The recovered purified enterocin has decreased procedure time and diminished the number of undesirable molecules present in Rogosa and Sharpe (MRS) broth. The recovered enterocin showed antimicrobial activity against several foodborne pathogenic and spoilage microbes. Conclusion:  The recovered enterocin was able to tolerate a variety of food chain conditions such as high temperature, pH and storage stability, and it can be a good candidate to protect food from spoilage microbes.  
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hal-03124447 , version 1 (28-01-2021)

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Imad Al Kassaa, Mazen Zaylaa, Samah Hussein, Khaled El Omari, Nour-Eddine Chihib, et al.. Identification of the first bacteriocin isolated in Lebanon extracted via a modified adsorption-desorption method and its potential food application. The International Arabic Journal of Antimicrobial Agents, 2018, The International Arabic Journal of Antimicrobial Agents, 8 (1), ⟨10.3823/817⟩. ⟨hal-03124447⟩
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