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BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants

Abstract : A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the lignification process, methodologies that simultaneously detect multiple chemical reporters in living organisms are still scarce. Here, we have developed an original bioorthogonal labeling imaging sequential strategy (BLISS) to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions. On our path to BLISS, we designed a new azide-tagged monolignol reporter for H units in metabolic lignin engineering and used it in conjunction with an alkyne-tagged G unit surrogate to study lignification dynamics in flax. Here, we show that BLISS provides precise spatial information on the zones of active lignification and reveals polarization in single-cell lignification dynamics.
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Soumis le : vendredi 12 mars 2021 - 13:37:31
Dernière modification le : mardi 19 octobre 2021 - 11:33:30

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Cedric Lion, Clémence Simon, Brigitte Huss, Anne-Sophie Blervacq, Louis Tirot, et al.. BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants. Cell Chemical Biology, Cell Press, 2017, Cell Chemical Biology, 24 (3), pp.326-338. ⟨10.1016/j.chembiol.2017.02.009⟩. ⟨hal-03167767⟩



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