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The expression and functional analysis of the sialyl-T antigen in prostate cancer

Abstract : Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of thecompositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer. In thisstudy, we used MALDI-TOF-MS analysis to determine theO-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived fromnormal prostatic tissue. Prostate cancer (CaP) cells exhibited an elevation of simple/shortO-glycans, with a reduction of complexO-glycans, increasedO-glycan sialylation and decreased fucosylation. Core 1 sialylation was increased dramatically in all CaPcells, and especially in PC-3 cells. The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product ofα2,3-sialyltransferase-I (ST3Gal-I) was substantially increased. We therefore focused on exploring the possible function ofST3Gal-I in PC-3 cells. ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migrationand apoptosis. Furtherin vivostudies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mousemodel, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be atarget for therapeutic intervention in cancer treatment.
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https://hal.univ-lille.fr/hal-03178789
Contributeur : Lilloa Université de Lille <>
Soumis le : mercredi 24 mars 2021 - 08:10:51
Dernière modification le : jeudi 25 mars 2021 - 03:32:19

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Ruifeng Bai, Xue Luan, Yu Zhang, Catherine Robbe, Inka Brockhausen, et al.. The expression and functional analysis of the sialyl-T antigen in prostate cancer. Glycoconjugate Journal, Springer Verlag, 2020, Glycoconjugate Journal, 37 (4), pp.423-433. ⟨10.1007/s10719-020-09927-x⟩. ⟨hal-03178789⟩

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