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Microbial diversity observed during hemp retting

Abstract : Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. This study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. We studied six samples harvested on different ground types, at different dates and with different retting durations on field to obtain an exhaustive representation of the process. After DNA extraction, total bacteria and fungi associated with stems during retting were specifically quantified using real-time PCR. Then, using sequence analysis of randomly cloned 16S and 18S ribosomal RNA (rRNA) genes, a phylogenetic characterisation of the dominant microorganisms was carried out. Quantitatively, we showed that there were 8.1–9.5 log10 16S rRNA gene copies per gram of hemp straw for bacteria and 8.6–9.6 log10 18S rRNA gene copies per gram for fungi. Qualitatively, we noticed a higher bacterial diversity in comparison to fungi. This work showed that in the different samples, the same species were present but in significantly different proportions according to ground type, harvest dates and retting durations on field. The most frequent bacterial sequences were affiliated to species Escherichia coli, Pantoea agglomerans, Pseudomonas rhizosphaerae, Rhodobacter sp., Pseudomonas fulva, Rhizobium huautlense and Massilia timonae, whereas fungal sequences were principally related to the genera Cladosporium and Cryptococcus.
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https://hal.univ-lille.fr/hal-03180897
Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 25 mars 2021 - 12:32:16
Dernière modification le : jeudi 22 avril 2021 - 03:20:46

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Alexandra Ribeiro, Philippe Pochart, Arnaud Day, Sarah Mennuni, Pierre Bono, et al.. Microbial diversity observed during hemp retting. Applied Microbiology and Biotechnology, Springer Verlag, 2015, Applied Microbiology and Biotechnology, 99 (10), pp.4471-4484. ⟨10.1007/s00253-014-6356-5⟩. ⟨hal-03180897⟩

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