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Synaptic protein changes after a chronic period of sensorimotor perturbation in adult rats: a potential role of phosphorylation/O-GlcNAcylation interplay.

Abstract : In human, a chronic sensorimotor perturbation (SMP) through prolonged body immobilization alters motor task performance through a combination of peripheral and central factors. Studies performed on a rat model of SMP have shown biomolecular changes and a reorganization of sensorimotor cortex through events such as morphological modifications of dendritic spines (number, length, functionality). However, underlying mechanisms are still unclear. It is well known that phosphorylation regulates a wide field of synaptic activity leading to neuroplasticity. Another post-translational modification that interplays with phosphorylation is O-GlcNAcylation. This atypical glycosylation, reversible, and dynamic, is involved in essential cellular and physiological processes such as synaptic activity, neuronal morphogenesis, learning, and memory. We examined potential roles of phosphorylation/O-GlcNAcylation interplay in synaptic plasticity within rat sensorimotor cortex after a SMP period. For this purpose, sensorimotor cortex synaptosomes were separated by sucrose gradient, in order to isolate a subcellular compartment enriched in proteins involved in synaptic functions. A period of SMP induced plastic changes at the pre- and post-synaptic levels, characterized by a reduction in phosphorylation (synapsin1, α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors (AMPAR) GluA2) and expression (synaptophysin, PSD-95, AMPAR GluA2) of synaptic proteins, as well as a decrease in MAPK/ERK42 activation. Expression levels of O-GlcNAc transferase/O-GlcNAcase enzymes was unchanged but we observed a specific reduction of synapsin1 O-GlcNAcylation in sensorimotor cortex synaptosomes. The synergistic regulation of synapsin1 phosphorylation/O-GlcNAcylation could affect pre-synaptic neurotransmitter release. Associated with other pre- and post-synaptic changes, synaptic efficacy could be impaired in somatosensory cortex of SMP rat. Thus, phosphorylation/O-GlcNAcylation interplay appears to be involved in synaptic plasticity by finely regulating neural activity.
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https://hal.univ-lille.fr/hal-03227650
Contributeur : Lilloa Université de Lille <>
Soumis le : lundi 17 mai 2021 - 14:43:21
Dernière modification le : mardi 18 mai 2021 - 03:37:56

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Julie Fourneau, Marie-Helene Canu, Caroline Cieniewski, Bruno Bastide, Erwan Dupont. Synaptic protein changes after a chronic period of sensorimotor perturbation in adult rats: a potential role of phosphorylation/O-GlcNAcylation interplay.. Journal of neurochemistry, 2018, Journal of neurochemistry, 147 (2), pp.240-255. ⟨10.1111/jnc.14474⟩. ⟨hal-03227650⟩

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