Accéder directement au contenu Accéder directement à la navigation
Chapitre d'ouvrage

The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein

Abstract : Nuclear magnetic resonance (NMR) spectroscopy can be used as an analytical tool to investigate posttranslational modifications of protein. NMR is a valuable tool to map the interaction regions of protein partners. Here, we present protocols that have been developed in the course of our studies of the neuronal Tau protein. Tau is found aggregated in the neurons of Alzheimer’s disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation and acetylation of Tau. We have used NMR to investigate how these posttranslational modifications of Tau affect the interactions with its partners. We present here detailed protocols of in vitro phosphorylation of Tau by recombinant kinase, ERK2, or kinase activity of rat brain extracts, and acetylation by recombinant Creb-binding protein (CBP) acetyltransferase. The analytical characterization of the modified Tau by NMR spectroscopy is additionally described.
Type de document :
Chapitre d'ouvrage
Liste complète des métadonnées

https://hal.univ-lille.fr/hal-03235823
Contributeur : Lilloa Université de Lille <>
Soumis le : mercredi 26 mai 2021 - 08:57:36
Dernière modification le : jeudi 27 mai 2021 - 03:35:34

Identifiants

Collections

Citation

Haoling Qi, Clément Despres, Sudhakaran Prabakaran, Francois-Xavier Cantrelle, Béatrice Chambraud, et al.. The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein. Nocca Smet, Caroline. Tau protein : methods and protocols, 1523, Springer New York, pp.179-213, 2017, 978-1-4939-6596-0. ⟨10.1007/978-1-4939-6598-4_11⟩. ⟨hal-03235823⟩

Partager

Métriques

Consultations de la notice

11