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Article dans une revue

Regulatory O-GlcNAcylation sites on FoxO1 are yet to be identified

Abstract : O-GlcNAcylation is a reversible post-translational modification that regulates cytosolic and nuclear proteins. We and others previously demonstrated that FoxO1 is O-GlcNAcylated in different cell types, resulting in an increase in its transcriptional activity. Four O-GlcNAcylation sites were identified in human FOXO1 but directed mutagenesis of each site individually had modest (T317) or no effect (S550, T648, S654) on its O-GlcNAcylation status and transcriptional activity. Moreover, the consequences of mutating all four sites had not been investigated. In the present work, we mutated these sites in the mouse Foxo1 and found that mutation of all four sites did not decrease Foxo1 O-GlcNAcylation status and transcriptional activity, and would even tend to increase them. In an attempt to identify other O-GlcNAcylation sites, we immunoprecipitated wild-type O-GlcNAcylated Foxo1 and analysed the tryptic digest peptides by mass spectrometry using High-energy Collisional Dissociation. We identified T646 as a new O-GlcNAcylation site on Foxo1. However, site directed mutagenesis of this site individually or together with all four previously identified residues did not impair Foxo1 O-GlcNAcylation and transcriptional activity. These results suggest that residues important for the control of Foxo1 activity by O-GlcNAcylation still remain to be identified.
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Soumis le : mardi 13 juillet 2021 - 09:45:13
Dernière modification le : mercredi 27 octobre 2021 - 16:14:02



Yann Fardini, Yobana Perez-Cervera, Luc Camoin, Patrick Pagesy, Tony Lefebvre, et al.. Regulatory O-GlcNAcylation sites on FoxO1 are yet to be identified. Biochemical and biophysical research communications, 2015, Biochemical and biophysical research communications, 462 (2), pp.151-158. ⟨10.1016/j.bbrc.2015.04.114⟩. ⟨hal-03285064⟩



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