Quantitative assessment of organ distribution of dietary protein-bound C-13-labeled N-epsilon-carboxymethyllysine after a chronic oral exposure in mice - Université de Lille Accéder directement au contenu
Article Dans Une Revue Molecular Nutrition and Food Research Année : 2016

Quantitative assessment of organ distribution of dietary protein-bound C-13-labeled N-epsilon-carboxymethyllysine after a chronic oral exposure in mice

Résumé

Scope Nɛ-Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found. Methods and results A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass-to-charge ratios were used: nCML Nε-carboxymethyl-L-lysine, dCML Nε-[13C]carboxy[13C]methyl-L-lysine and internal standard Nε-carboxymethyl-L-[4,4,5,5-2H4]lysine. Wild-type (n = 7) and RAGE−/− (n = 8) mice were fed for 30 days with either a control, or a BSA-bound dCML-enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography–tandem mass spectrometry. Mice exposed to dCML showed an accumulation in all tissues tested except fat. The rate of deposition was high (81–320 μgdCML/g dry matter) in kidneys, intestine, and lungs and low (<5 μg/g) in heart, muscle, and liver. This accumulation was not RAGE dependent. Conclusion The kidney is not the only organ affected by the accumulation of dCML. Its high accumulation in other tissues and organs may also, however, have important physiological consequences.

Dates et versions

hal-04064620 , version 1 (11-04-2023)

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Citer

Frederic Tessier, Celine Niquet-Leridon, Philippe Jacolot, Celine Jouquand, Michaël Genin, et al.. Quantitative assessment of organ distribution of dietary protein-bound C-13-labeled N-epsilon-carboxymethyllysine after a chronic oral exposure in mice. Molecular Nutrition and Food Research, 2016, Molecular nutrition & food research, 60 (11), pp.2446-2456. ⟨10.1002/mnfr.201600140⟩. ⟨hal-04064620⟩
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