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Article Dans Une Revue Membranes Année : 2020

Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification.

Résumé

The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.
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hal-04211907 , version 1 (20-09-2023)

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Rémi Przybylski, Laurent Bazinet, Loubna Firdaous, Mostafa Kouach, Jean-Francois Goossens, et al.. Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification.. Membranes, 2020, Membranes (Basel), 10 (5), pp.90. ⟨10.3390/membranes10050090⟩. ⟨hal-04211907⟩
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