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Chapitre D'ouvrage Année : 2020

Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro

Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)

Résumé

Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes. In the virus-induced gene silencing (VIGS) method, it is possible to take advantage of this mechanism by inserting a plant nucleic fragment within the viral genome to knock down the corresponding gene. This tool has been used in many species as a fast and easy reverse genetics technique in order to gain information on the role of genes with poorly understood functions. Here we describe in detail two Agrobacterium-mediated infection protocols in flax, based on a whole plant vacuum infiltration and a leaf syringe infiltration that systemically impact the transcript levels in the stem.
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hal-04231129 , version 1 (10-10-2023)

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Oriane Morel, Corentin Spriet, Cédric Lion, Fabien Baldacci-Cresp, Garance Pontier, et al.. Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro. Virus-Induced Gene Silencing in Plants Methods and Protocols, Springer US; Vincent Courdavault and Sébastien Besseau / Humana Press, pp.65-74, 2020, ⟨10.1007/978-1-0716-0751-0_6⟩. ⟨hal-04231129⟩

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