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Article Dans Une Revue Clinical and experimental obstetrics & gynecology Année : 2020

Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed?

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Objective: To determine if a model of acidosis caused by occlusion of the umbilical cord could be repeated in the same sheep in ethicaly order to reduce the number of animals needed. Method: To obtain fetal acidosis, 1 min of total umbilical cord occlusion (UCO) every 2.5 min was performed on fetal sheep (n=7) until pH reached 7.10. Chronic instrumentation regularly recorded arterial blood gas and hemodynamic parameters. The occlusions were repeated to compare the data between the 2 days for each animal. Results: At the end of the UCO period, fetal acidosis was obtained with a pH 7.14 on day 1 (D1) and 7.06 on day 2 (D2). At baseline, fetuses were more hypotensive at D2 (41 mmHg vs 44 mmHg on D1, p < 0.05). During the UCO period, there was no significant difference in blood gas and hemodynamic data between D1 and D2. Conclusion: To reduce the number of animals needed, performing a second experiment on the same animal appears possible even if results must be interpreted cautiously when severe acidosis is reached.
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hal-04344538 , version 1 (14-12-2023)

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Clementine Pierre, Louise Ghesquiere, Julien de Jonckheere, Estelle Aubry, Dyuti Sharma, et al.. Fetal acidosis in a sheep model: can we perform a second day of protocol in order to reduce the numbers of animals needed?. Clinical and experimental obstetrics & gynecology, 2020, Clinical and experimental obstetrics & gynecology, 47 (1), pp.79-83. ⟨10.31083/j.ceog.2020.01.5120⟩. ⟨hal-04344538⟩

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