MET exon 14 skipping mutation is a hepatocyte growth factor (HGF)-dependent oncogenic driver in vitro and in humanized HGF knock-in mice. - Université de Lille Accéder directement au contenu
Article Dans Une Revue Molecular Oncology Année : 2023

MET exon 14 skipping mutation is a hepatocyte growth factor (HGF)-dependent oncogenic driver in vitro and in humanized HGF knock-in mice.

Marie Fernandes
B. Hoggard
  • Fonction : Auteur
C. Descarpentries
  • Fonction : Auteur
J. Stanislovas
  • Fonction : Auteur
E. Farage
  • Fonction : Auteur
K. Bouchekioua-Bouzaghou
  • Fonction : Auteur
M. Nollet
  • Fonction : Auteur
T. Perera
  • Fonction : Auteur
P. Fromme
  • Fonction : Auteur
S. Kermorgant
  • Fonction : Auteur

Résumé

Exon skipping mutations of the MET receptor tyrosine kinase (METex14), increasingly reported in cancers, occur in 3–4% of non–small-cell lung cancer (NSCLC). Only 50% of patients have a beneficial response to treatment with MET-tyrosine kinase inhibitors (TKIs), underlying the need to understand the mechanism of METex14 oncogenicity and sensitivity to TKIs. Whether METex14 is a driver mutation and whether it requires hepatocyte growth factor (HGF) for its oncogenicity in a range of in vitro functions and in vivo has not been fully elucidated from previous preclinical models. Using CRISPR/Cas9, we developed a METex14/WT isogenic model in nontransformed human lung cells and report that the METex14 single alteration was sufficient to drive MET-dependent in vitro anchorage-independent survival and motility and in vivo tumorigenesis, sensitising tumours to MET-TKIs. However, we also show that human HGF (hHGF) is required, as demonstrated in vivo using a humanised HGF knock-in strain of mice and further detected in tumour cells of METex14 NSCLC patient samples. Our results also suggest that METex14 oncogenicity is not a consequence of an escape from degradation in our cell model. Thus, we developed a valuable model for preclinical studies and present results that have potential clinical implication.
Fichier principal
Vignette du fichier
Molecular Oncology - 2023 - Fernandes - MET exon 14 skipping mutation is a hepatocyte growth factor HGF ‐dependent.pdf (2.8 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-04405493 , version 1 (19-01-2024)

Licence

Identifiants

Citer

Marie Fernandes, B. Hoggard, Philippe Jamme, Sonia Paget, Marie-Jose Truong, et al.. MET exon 14 skipping mutation is a hepatocyte growth factor (HGF)-dependent oncogenic driver in vitro and in humanized HGF knock-in mice.. Molecular Oncology, 2023, Molecular Oncology, 17 (11), p. 2257-2274. ⟨10.1002/1878-0261.13397⟩. ⟨hal-04405493⟩
27 Consultations
11 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More