Proteomic and Phosphoproteomic Changes Induced by Prolonged Activation of Human Eosinophils with IL-3. - Université de Lille Accéder directement au contenu
Article Dans Une Revue Journal of Proteome Research Année : 2018

Proteomic and Phosphoproteomic Changes Induced by Prolonged Activation of Human Eosinophils with IL-3.

Alexander S Hebert
  • Fonction : Auteur
Nizar N Jarjour
  • Fonction : Auteur
Joshua J Coon
  • Fonction : Auteur
Deane F Mosher
  • Fonction : Auteur

Résumé

Purified human eosinophils treated for 18-24 h with IL-3 adopt a unique activated phenotype marked by increased reactivity to aggregated immunoglobulin-G (IgG). To characterize this phenotype, we quantified protein abundance and phosphorylation by multiplexed isobaric labeling combined with high-resolution mass spectrometry. Purified blood eosinophils of five individuals were treated with IL-3 or no cytokine for 20 h, and comparative data were obtained on abundance of 5385 proteins and phosphorylation at 7330 sites. The 1150 proteins that were significantly up-regulated ( q < 0.05, pairwise t test with Benjamini-Hochberg correction) by IL-3 included the IL3RA and CSF2RB subunits of the IL-3 receptor, the low-affinity receptor for IgG (FCGR2B), 96 proteins involved in protein translation, and 55 proteins involved in cytoskeleton organization. Among the 703 proteins that decreased were 78 mitochondrial proteins. Dynamic regulation of protein phosphorylation was detected at 4218 sites. These included multiple serines in CSF2RB; Y694 of STAT5, a key site of activating phosphorylation downstream of IL3RA/CSF2RB; and multiple sites in RPS6KA1, RPS6, and EIF4B, which are responsible for translational initiation. We conclude that IL-3 up-regulates overall protein synthesis and targets specific proteins for up-regulation, including its own receptor.
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Dates et versions

hal-04507246 , version 1 (16-03-2024)

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Stéphane Esnault, Alexander S Hebert, Nizar N Jarjour, Joshua J Coon, Deane F Mosher. Proteomic and Phosphoproteomic Changes Induced by Prolonged Activation of Human Eosinophils with IL-3.. Journal of Proteome Research, 2018, Journal of Proteome Research, 17, pp.2102-2111. ⟨10.1021/acs.jproteome.8b00057⟩. ⟨hal-04507246⟩

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