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Chapitre D'ouvrage Année : 2021

Observation and Quantification of Eosinophil Motility

Karina T Barretto
  • Fonction : Auteur
Deane F Mosher
  • Fonction : Auteur
Nizar N Jarjour
  • Fonction : Auteur
Colleen S Curran
  • Fonction : Auteur
Mats W Johansson
  • Fonction : Auteur

Résumé

Eosinophils are important for tissue homeostasis and host responses to pathogens and allergens. The impact of eosinophils within tissues depends in part on whether cytotoxic proteins in crystalloid granules are released. Determinants of eosinophil motility and loss of granule contents are incompletely understood. The goal of this chapter is to present methods to study the effects of potential mediators on purified human blood eosinophils interacting with adhesive proteins found in extracellular matrix. We show that differential interference contrast video-enhanced microscopy and a bead-clearing assay provide complementary information about how different mediator-adhesive protein combinations direct eosinophil motility and granule fate. The former method is rich in information about cell shape, pattern of movement, and state of granules whereas the latter method lends itself to quantification and interrogation of multiple conditions in replicate.
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Dates et versions

hal-04509034 , version 1 (18-03-2024)

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Citer

Karina T Barretto, Deane F Mosher, Nizar N Jarjour, Colleen S Curran, Stéphane Esnault, et al.. Observation and Quantification of Eosinophil Motility. Eosinophils, Springer Verlag, pp.139-148, 2021, ⟨10.1007/978-1-0716-1095-4_12⟩. ⟨hal-04509034⟩

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