Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices. - Université de Lille Accéder directement au contenu
Article Dans Une Revue STAR Protocols Année : 2023

Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices.

Résumé

Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes of the arcuate nucleus of the hypothalamus and pro-opiomelanocortin neurons, this protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.

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Neurosciences
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hal-04578887 , version 1 (17-05-2024)

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Tori Lhomme, Vincent Prevot. Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices.. STAR Protocols, 2023, STAR Protocols, 4, pp.102571. ⟨10.1016/j.xpro.2023.102571⟩. ⟨hal-04578887⟩

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