IL-3 up-regulates and activates human eosinophil CD32 and αMβ2 integrin causing degranulation. - Université de Lille Accéder directement au contenu
Article Dans Une Revue Clinical & Experimental Allergy Année : 2017

IL-3 up-regulates and activates human eosinophil CD32 and αMβ2 integrin causing degranulation.

M W Johansson
  • Fonction : Auteur
E A Kelly
  • Fonction : Auteur
L Koenderman
  • Fonction : Auteur
D F Mosher
  • Fonction : Auteur
N N Jarjour
  • Fonction : Auteur

Résumé

Eosinophils contribute to the pathogenesis of multiple diseases, including asthma. Treatment with antibodies targeting IL-5 or IL-5 receptor α reduces the frequency of asthma exacerbations. Eosinophil receptors for IL-5 share a common ß-chain with IL-3 and GM-CSF receptors. We recently reported that IL-3 is more potent than IL-5 or GM-CSF in maintaining the ERK/p90S6K/RPS6 ribosome-directed signaling pathway, leading to increased protein translation. We aimed to determine disease-relevant consequences of prolonged eosinophil stimulation with IL-3. Human blood eosinophils were used to establish the impact of activation with IL-3 on IgG-driven eosinophil degranulation. When compared to IL-5, continuing exposure to IL-3 further induced degranulation of eosinophils on aggregated IgG via increased production and activation of both CD32 (low affinity IgG receptor) and αMß2 integrin. In addition, unlike IL-5 or GM-CSF, IL-3 induced expression of CD32B/C (FCGRIIB/C) subtype proteins, without changing CD32A (FCGRIIA) protein and CD32B/C mRNA expression levels. Importantly, these in vitro IL-3-induced modifications were recapitulated in vivo on airway eosinophils. We observed for the first time upregulation of CD32B/C on eosinophils, and identified IL-3 as a potent inducer of CD32- and αMß2-mediated eosinophil degranulation.
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Dates et versions

hal-04614223 , version 1 (17-06-2024)

Identifiants

  • HAL Id : hal-04614223 , version 1
  • PUBMED : 28000949

Citer

Stéphane Esnault, M W Johansson, E A Kelly, L Koenderman, D F Mosher, et al.. IL-3 up-regulates and activates human eosinophil CD32 and αMβ2 integrin causing degranulation.. Clinical & Experimental Allergy, 2017, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 47, pp.488-498. ⟨hal-04614223⟩

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