Small molecule MarR modulators potentiate metronidazole antibiotic activity in aerobic E. coli by inducing activation by the nitroreductase NfsA. - Université de Lille Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2024

Small molecule MarR modulators potentiate metronidazole antibiotic activity in aerobic E. coli by inducing activation by the nitroreductase NfsA.

Résumé

Antibiotic resistant Enterobacterales pose a major threat to healthcare systems worldwide, necessitating the development of novel strategies to fight such hard-to-kill bacteria. One potential approach is to develop molecules that force bacteria to hyper-activate prodrug antibiotics, thus rendering them more effective. In the present work, we aimed to obtain proof-of-concept data to support that small molecules targeting transcriptional regulators can potentiate the antibiotic activity of the prodrug metronidazole (MTZ) against Escherichia coli under aerobic conditions. By screening a chemical library of small molecules a series of structurally related molecules were identified that had little inherent antibiotic activity, but showed substantial activity in combination with ineffective concentrations of MTZ. Transcriptome analyses, functional genetics, thermal shift assays and electrophoretic mobility shift assays were then used to demonstrate that these MTZ boosters target the transcriptional repressor MarR, resulting in the up-regulation of the marRAB operon and its downstream MarA regulon. The associated upregulation of the flavin-containing nitro-reductase; NfsA was then shown to be critical for the booster mediated potentiation of MTZ antibiotic activity. Transcriptomic studies, biochemical assays and electron paramagnetic resonance measurements were then used to show that under aerobic conditions, NfsA catalyzed 1-electron reduction of MTZ to the MTZ radical anion which in turn induced lethal DNA damage in E. coli. This work reports the first example of prodrug boosting in Enterobacterales by transcriptional modulators and highlights that MTZ antibiotic activity can be chemically induced under anaerobic growth conditions.
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Dates et versions

hal-04625214 , version 1 (26-06-2024)

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Thibault Caradec, Coline Ple, Giuseppe Sicoli, Ravil Petrov, Elizabeth Pradel, et al.. Small molecule MarR modulators potentiate metronidazole antibiotic activity in aerobic E. coli by inducing activation by the nitroreductase NfsA.. Journal of Biological Chemistry, 2024, J Biol Chem, pp.107431. ⟨10.1016/j.jbc.2024.107431⟩. ⟨hal-04625214⟩
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