Article Dans Une Revue RSC Chemical Biology Année : 2024

Click-ready iridium(iii) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling

Résumé

Herein, we report the synthesis, photophysical characterization and validation of iridium(III)–polypyridine complexes functionalized for click chemistry and bioorthogonal chemistry, as well as their versatile applications as probes in bioimaging studies exploiting metabolic labeling. The designed dyes are conjugated to chemical reporters in a specific manner within cells by CuAAC ligation and display attractive photophysical properties in the UV-visible range. They are indeed highly photostable and emit in the far-red to near-IR region with long lifetimes and large Stokes shifts. We demonstrate that they can be efficiently used to monitor nascent intracellular sialylated glycoconjugates in bioorthogonal MOE studies with a varied panel of optical and non-optical techniques, namely conventional UV-vis laser scanning confocal microscopy (for routine purposes), UV-vis time-resolved luminescence imaging (for specificity and facilitated multiplexing with nano-environment sensitivity), synchrotron radiation based X-ray fluorescence nanoimaging (for high resolution, elemental mapping and quantification in situ) and inductively coupled plasma mass spectrometry (for routine quantification on cell populations with high statistical confidence). The synthesized Ir(III) complexes were utilized in single labeling experiments, as well as in dual click-labeling experiments utilizing two distinct monosaccharide reporters relevant to the same metabolic pathway.
Fichier principal
Vignette du fichier
d4cb00255e.pdf (3.04 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04887582 , version 1 (15-01-2025)

Licence

Identifiants

Citer

Vincent Rigolot, Clemence Simon, Aude Bouchet, Lucas Lancel, V. Di Battista, et al.. Click-ready iridium(iii) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling. RSC Chemical Biology, 2024, RSC Chem. Biol., -, ⟨10.1039/d4cb00255e⟩. ⟨hal-04887582⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More