Article Dans Une Revue STAR Protocols Année : 2024

Protocol for generating a co-culture of macrophages with breast cancer tumoroids.

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Cancer progression and treatment outcomes are heavily influenced by the tumor microenvironment (TME), especially through immune cell interactions. Here, we present a protocol for generating co-cultures of tumoroids with macrophages, either semi-liquid or Matrigel-embedded. We describe steps for macrophage preparation, co-culture establishment, and medium adjustments to support cell viability and function. While optimized for breast cancer models, this protocol can be adapted to other tumor types with appropriate medium adjustments.
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hal-04918128 , version 1 (29-01-2025)

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Antonella Raffo Romero, Lydia Ziane Chaouche, Nawale Hajjaji, Michel Salzet, Marie Duhamel. Protocol for generating a co-culture of macrophages with breast cancer tumoroids.. STAR Protocols, 2024, STAR Protocols, 6 (1), pp.103536. ⟨10.1016/j.xpro.2024.103536⟩. ⟨hal-04918128⟩

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