Transgenic Mouse Reporter to Study Muc5b In Vivo.

Jean-Luc Desseyn 1 Céline Portal Frederic Gottrand 1 Valérie Gouyer 1
1 LIRIC - Lille Inflammation Research International Center
Abstract : Dysregulation of gel-forming mucins is associated with many airway diseases. Better knowledge of the pathophysiological mechanisms linking mucins and respiratory diseases will advance the understanding of their pathogenesis and should provide opportunities to develop new therapeutic compounds for treatment. MUC5B and MUC5AC are the two main gel-forming mucins in the respiratory tract. The organization in domains and the expression profile of mouse Muc5b are very similar to those in humans, which makes the mouse a relevant model for studies of the translational activities of human mucins. To assess the in vivo biological functions of Muc5b, a mouse reporter tagged in frame with the green fluorescent protein marker has been engineered by homologous recombination. The proof of concept that this reporter model may be informative for translational studies was confirmed by the finding that interleukin-13 administration in living mice upregulated Muc5b production.
Keywords : mucus Muc5b–green fluorescent protein green fluorescent protein live imaging interleukin-13 mucins
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Soumis le : jeudi 6 juin 2019 - 14:01:35
Dernière modification le : jeudi 12 septembre 2019 - 17:31:42

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Jean-Luc Desseyn, Céline Portal, Frederic Gottrand, Valérie Gouyer. Transgenic Mouse Reporter to Study Muc5b In Vivo.. Annals of the American Thoracic Society, American Thoracic Society, 2018, Annals of the American Thoracic Society, 15, pp.S149-S153. ⟨10.1513/AnnalsATS.201802-085AW⟩. ⟨hal-02149469⟩

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