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Abnormal expression of Muc5b in Cftr-null mice and in mammary tumors of MMTV-ras mice.

Abstract : Gel-forming mucins are large, high molecular weight, and heavily O-glycosylated proteins that are responsible for the rheological properties of mucus gel. Among them, the mucin MUC5B has been implicated in breast cancer and cystic fibrosis. We obtained a new polyclonal serum, named CP1, which was isolated from a rabbit immunized with a mouse Muc5b peptide. The immunoprofile of Muc5b was determined on paraffin-embedded and frozen mouse tissue sections and showed a similar expression pattern in mouse to that in the human. The "nonmammary" mucin Muc5b was detected in all mammary tumors analyzed from MMTV-ras mice, suggesting that the CP1 antibody is a valuable tool for investigating the involvement of this mucin in mammary cancer. We also found that uninfected Cftr( -/- ) mice harbored more Clara cells, which were Muc5b-positive, than did their wild-type control littermates. The number of Muc5b-positive cells increased in Cftr( -/- ) mice infected experimentally with Pseudomonas aeruginosa, and the mice developed mucus plugs in their bronchi and bronchioles with a high frequency of Muc5b content (87%, Cohen's kappa = 0.82; p < 0.0001). These findings suggest that mice genetically deficient in the Cftr gene are predisposed to develop mucus plugs and that MUC5B may provide a valuable target for decreasing mucus viscosity in cystic fibrosis.
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https://hal.univ-lille.fr/hal-02176612
Contributeur : Lilloa Université de Lille <>
Soumis le : lundi 8 juillet 2019 - 12:56:23
Dernière modification le : mercredi 20 novembre 2019 - 13:48:56

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Hélène Valque, Valérie Gouyer, Marie-Odile Husson, Frederic Gottrand, Jean-Luc Desseyn. Abnormal expression of Muc5b in Cftr-null mice and in mammary tumors of MMTV-ras mice.. Histochemistry and Cell Biology, Springer Verlag, 2011, 136 (6), pp.699-708. ⟨10.1007/s00418-011-0872-5⟩. ⟨hal-02176612⟩

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