Long-term dietary (n-3) polyunsaturated fatty acids show benefits to the lungs of Cftr F508del mice.

Celine Portal 1 Valérie Gouyer 1 Renaud Leonard 2 Marie-Odile Husson 1 Frederic Gottrand 1 Jean Luc Desseyn 1
1 LIRIC - Lille Inflammation Research International Center
2 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576
Abstract : INTRODUCTION: The pro-inflammatory status of cystic fibrosis (CF) patients promotes pulmonary colonization with opportunist and pathogenic bacteria, which is favored by a sticky mucus. Oral supplementation with (n-3) long chain polyunsaturated fatty acids (LC-PUFA) has shown anti-inflammatory effects. The aim of this study was to demonstrate the positive effects of a long-term diet enriched in (n-3) LC-PUFA on the lungs of Cftr F508del mice. MATERIALS AND METHODS: Breeding CftrDeltaF508del/+ mice received a control diet or a diet enriched in (n-3) LC-PUFA for 5 weeks before mating, gestation and lactation. After weaning, the offspring were given the same diet as their mother until post-natal day 60. The effects of (n-3) LC-PUFA supplementation on the lungs were evaluated in homozygous Cftr F508del mice and their wild-type littermates after acute lung inflammation induced by Pseudomonas aeruginosa lipopolysaccharide (LPS) inhalation. RESULTS: (n-3) LC-PUFA enrichment of mothers contributes to enrichment of mammary milk and cell membrane of suckling pups. Cftr F508del mice exhibited growth retardation and lung damage with collapsed alveoli, hyperplasia of bronchial epithelial cells and inflammatory cell infiltration. The (n-3) LC-PUFA diet corrected the growth delay of Cftr F508del mice and decreased hyperplasia of bronchial epithelial cells. Besides decreasing metaplasia of Club cells after LPS inhalation, (n-3) LC-PUFA modulated lung inflammation and restricted lung damage. CONCLUSION: Long-term (n-3) LC-PUFA supplementation shows moderate benefits to the lungs of Cftr F508del mice.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [43 références]  Voir  Masquer  Télécharger
https://hal.univ-lille.fr/hal-02361429
Contributeur : Lilloa Université de Lille <>
Soumis le : mercredi 13 novembre 2019 - 13:30:24
Dernière modification le : samedi 16 novembre 2019 - 01:26:04

Fichier

Accepted_Manuscript_clean.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

UNIV-LILLE | INRA | RIIP | RIIP_LILLE | CNRS

Citation

Celine Portal, Valérie Gouyer, Renaud Leonard, Marie-Odile Husson, Frederic Gottrand, et al.. Long-term dietary (n-3) polyunsaturated fatty acids show benefits to the lungs of Cftr F508del mice.. PLoS ONE, Public Library of Science, 2018, PLoS One, 13, pp.e0197808. ⟨10.1371/journal.pone.0197808⟩. ⟨hal-02361429⟩

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

9

Téléchargements de fichiers

22

Contact

Informations

CCSD