Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Increasing O-GlcNAcylation level on organ culture of soleus modulates the calcium activation parameters of muscle fibers.

Abstract : O-N-acetylglucosaminylation is a reversible post-translational modification which presents a dynamic and highly regulated interplay with phosphorylation. New insights suggest that O-GlcNAcylation might be involved in striated muscle physiology, in particular in contractile properties such as the calcium activation parameters. By the inhibition of O-GlcNAcase, we investigated the effect of the increase of soleus O-GlcNAcylation level on the contractile properties by establishing T/pCa relationships. We increased the O-GlcNAcylation level on soleus biopsies performing an organ culture of soleus treated or not with PUGNAc or Thiamet-G, two O-GlcNAcase inhibitors. The enhancement of O-GlcNAcylation pattern was associated with an increase of calcium affinity on slow soleus skinned fibers. Analysis of the glycoproteins pattern showed that this effect is solely due to O-GlcNAcylation of proteins extracted from skinned biopsies. We also characterized the O-GlcNAcylated contractile proteins using a proteomic approach, and identified among others troponin T and I as being O-GlcNAc modified. We quantified the variation of O-GlcNAc level on all these identified proteins, and showed that several regulatory contractile proteins, predominantly fast isoforms, presented a drastic increase in their O-GlcNAc level. Since the only slow isoform of contractile protein presenting an increase of O-GlcNAc level was MLC2, the effect of enhanced O-GlcNAcylation pattern on calcium activation parameters could involve the O-GlcNAcylation of sMLC2, without excluding that an unidentified O-GlcNAc proteins, such as TnC, could be potentially involved in this mechanism. All these data strongly linked O-GlcNAcylation to the modulation of contractile activity of skeletal muscle.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [42 références]  Voir  Masquer  Télécharger

https://hal.univ-lille.fr/hal-02375641
Contributeur : Lilloa Université de Lille <>
Soumis le : mardi 12 mai 2020 - 14:47:46
Dernière modification le : vendredi 15 mai 2020 - 02:23:58

Fichier

2012_Cieniewski-Bernard_Plos O...
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

  • HAL Id : hal-02375641, version 3
  • PUBMED : 23110217

Collections

Citation

Caroline Cieniewski, Valerie Montel, Serge Berthoin, Bruno Bastide. Increasing O-GlcNAcylation level on organ culture of soleus modulates the calcium activation parameters of muscle fibers.. PLoS ONE, Public Library of Science, 2012, PLoS One, 7, pp.e48218. ⟨hal-02375641v3⟩

Partager

Métriques

Consultations de la notice

15

Téléchargements de fichiers

24