Self-stratification is an innovative process providing a multi functional coating. The concept of self-stratifying coating is to bring the primer, intermediate and top coat properties together in a one-pot formulation. To design a self-stratifying coating, two partially incompatible resins are mixed in a solvent blend which separates spontaneaously after application and curing. To compare to the traditional multi-layer coating approach, self-stratification allows reducing the number of formulation steps, chemicals used and pollution emission. However, designing a perfectly stratified coating is a challenge, taking into account the high number of parameters involved (solvents, curing temperature, viscosity, etc.). Moreover, most already designed self-stratifying coatings use oil-based epoxy resins as thermoset resin, and “greener” solutions need to be investigated. In this work, bio-based epoxy resins and silicone resins were dissolved in various solvent blends and cured under different conditions. The stratification degree and the adhesion properties on a composite substrate were investigated depending on following parameters: (i) resin properties, (ii) cross-linking epoxy rate, (iii) curing temperature and (iv) solvent evaporation rate.