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Potential regulation of human muscle plasticity by MLC2 post-translational modifications during bed rest and countermeasures.
Abstract : This study investigated the effects of a 60-day bed rest with or without countermeasures on muscular phenotype and post-translational modifications of the regulatory Myosin Light Chain 2 (MLC2) protein. Soleus biopsies were obtained from female subjects before and after bed rest. Control subjects were assigned only to bed rest (BR), BR+Ex subjects were submitted to combined aerobic and resistive exercises, and BR+Nut to nutritional leucine and valine diet. We determined Myosin Heavy Chains (MHC) and MLC2 composition of muscles using 1D SDS-PAGE. MLC2 phosphorylation was measured on 2D gels and O-N-Acetyl Glucosaminylation (O-GlcNAc) level of MLC2 was determined. Our results showed a slow-to-fast shift of MHC and MLC2 isoforms in BR and BR+Nut while BR+Ex combinations prevented these phenotype changes. After BR, the MLC2 phosphorylation state was increased while the global MLC2 glycosylation level was decreased. Exercises prevented the variations of phosphorylation and glycosylation observed after BR whereas nutrition had no effects. These results suggested an interplay between phosphorylation and glycosylation of MLC2, which might be involved in the development of muscle atrophy and associated changes. These findings of differential responses to exercises and nutrition protocols were discussed with implications for future prescription models to preserve muscle against long-term unloading.
Keywords :
Adult
Bed Rest
Cardiac Myosins
Exercise
Female
Gene Expression Regulation
Glycosylation
Humans
Hypertrophy
Muscle
Skeletal
Muscular Atrophy
Myosin Light Chains
Phenotype
Phosphorylation
Protein Processing
Post-Translational
Countermeasures
Human soleus
Inactivity
Myosin Light Chain 2
O-glycosylation
https://hal.univ-lille.fr/hal-02917011
Contributeur : Lilloa Université de Lille <>
Soumis le : mardi 18 août 2020 - 13:32:54
Dernière modification le : lundi 26 avril 2021 - 17:46:08
Contributeur : Lilloa Université de Lille <>
Soumis le : mardi 18 août 2020 - 13:32:54
Dernière modification le : lundi 26 avril 2021 - 17:46:08