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Chapitre d'ouvrage

FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence

Abstract : Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of these reporters, which are now within the arsenal of biosensors, were devoted to the detection and characterization of the activity of the cAMP-Protein kinase A pathway. Based on the principle of this biosensor, other FRET-based kinase activity reporters emerged. Here, the choice of the kinase to be monitored, the artifacts that might be met, and the flexibility and amenability of the FRET-based kinase activity reporters both for high-throughput analysis and dissection of protein kinase functions are discussed.
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Chapitre d'ouvrage
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Contributeur : Lilloa Université de Lille <>
Soumis le : jeudi 7 janvier 2021 - 11:58:44
Dernière modification le : jeudi 21 janvier 2021 - 03:20:47


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Corentin Spriet, Angelina Kasprowicz, Dave Trinel, Jean-Francois Bodart. FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence. Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis, InTech, 2018, 978-953-51-3916-4. ⟨10.5772/intechopen.72388⟩. ⟨hal-03007678v2⟩



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