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Chapitre D'ouvrage Année : 2018

FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence

Résumé

Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of these reporters, which are now within the arsenal of biosensors, were devoted to the detection and characterization of the activity of the cAMP-Protein kinase A pathway. Based on the principle of this biosensor, other FRET-based kinase activity reporters emerged. Here, the choice of the kinase to be monitored, the artifacts that might be met, and the flexibility and amenability of the FRET-based kinase activity reporters both for high-throughput analysis and dissection of protein kinase functions are discussed.
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hal-03007678 , version 1 (16-11-2020)
hal-03007678 , version 2 (07-01-2021)

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Corentin Spriet, Angelina Kasprowicz, Dave Trinel, Jean-Francois Bodart. FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence. Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis, InTech, 2018, 978-953-51-3916-4. ⟨10.5772/intechopen.72388⟩. ⟨hal-03007678v2⟩

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