Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Detection and identification of O-GlcNAcylated proteins by proteomic approaches

Abstract : O-GlcNAcylation (O-linked beta-N-acetylglucosaminylation) is a widespread PTM confined within the nuclear, the cytosolic, and the mitochondrial compartments of eukaryotes. Recently, O-GlcNAcylation has been also detected in the close vicinity of plasma membranes particularly in lipid microdomains. The detection of this PTM can be easily done if appropriate controls and precautions are taken using a wide variety of tools including lectins, antibodies, or click-chemistry-based methods. In contrast, the identification of the proteins bearing O-GlcNAc moieties and the localization of the precise sites of O-GlcNAcylation remain challenging. This is due to the lability of the glycosidic bond between hydroxyl group of serine or threonine and N-acetylglucosamine using conventional fragmentation techniques such as CID. To tentatively overcome this technical limitation, electron-capture dissociation, or electron-transfer dissociation MS/MS are now used. Thanks to these breakthroughs, a large number of O-GlcNAc sites have been identified to date but these methodologies remain far from being used in routine.
Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Lilloa Université de Lille Connectez-vous pour contacter le contributeur
Soumis le : jeudi 18 mars 2021 - 13:16:40
Dernière modification le : mercredi 13 octobre 2021 - 10:34:02

Lien texte intégral




Anne-Sophie Vercoutter-Edouart, Ikram El Yazidi-Belkoura, Celine Guinez, Steffi F. Baldini, Maïté Leturcq, et al.. Detection and identification of O-GlcNAcylated proteins by proteomic approaches. Proteomics, 2015, Proteomics, 15 (5-6), pp.1039-1050. ⟨10.1002/pmic.201400326⟩. ⟨hal-03173280⟩



Consultations de la notice