A motif in the 5'untranslated region of messenger RNAs regulates protein synthesis in a S6 kinase-dependent manner. - Université de Lille Accéder directement au contenu
Article Dans Une Revue Advances in biological regulation Année : 2023

A motif in the 5'untranslated region of messenger RNAs regulates protein synthesis in a S6 kinase-dependent manner.

Résumé

The 5' untranslated regions (UTRs) in messenger RNAs (mRNAs) play an important role in the regulation of protein synthesis. We had previously identified a group of mRNAs that includes human semaphorin 7A (SEMA7A) whose translation is upregulated by the Erk/p90S6K pathway in human eosinophils, with a potential negative impact in asthma and airway inflammation. In the current study, we aimed to find a common 5'UTR regulatory cis-element, and determine its impact on protein synthesis. We identified a common and conserved 5'UTR motif GGCTG-[(C/G)T(C/G)]-GCC that was present in this group of mRNAs. Mutations of the first two GG bases in this motif in SEMA7A 5'UTR led to a complete loss of S6K activity dependence for maximal translation. In conclusion, the newly identified 5'UTR motif present in SEMA7A has a critical role in regulating S6K-dependent protein synthesis.
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Dates et versions

hal-04275074 , version 1 (08-11-2023)

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Hyun-Chul Shin, Yury A Bochkov, Kangsan Kim, James E Gern, Nizar N Jarjour, et al.. A motif in the 5'untranslated region of messenger RNAs regulates protein synthesis in a S6 kinase-dependent manner.. Advances in biological regulation, 2023, Advances in biological regulation, 89, pp.100975. ⟨10.1016/j.jbior.2023.100975⟩. ⟨hal-04275074⟩

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