In order to study the influence of human body parts on the overall comfort under different sports conditions, this paper designed a series of actions such as standing, squatting, running, walking, and so on, and obtained the key parts that affected the overall comfort at every experimental stage (i.e. every motion state) through subjective evaluation. That is, to study and analyze the comfort evaluation of every part and the whole body under different motions conditions, as well as the main parts that affect the overall comfort. In this paper, Analytic Hierarchy Process-Entropy weight, Fuzzy-Rough Set Theory, Analytic Hierarchy Process-Structural Equation Model, and Particle Swarm Optimization-Cuckoo Search were used to optimize the position index. At last, the prediction model of overall comfort was established by Adaptive Network-based Fuzzy Influence System. The input parameters are body part indexes screened by Analytic Hierarchy Process-Entropy weight, Fuzzy-Rough Set Theory, Analytic Hierarchy Process-Structural Equation Model and Particle Swarm Optimization-Cuckoo Search, respectively. And the output is the overall comfort evaluation value. Compared with the real value of overall comfort in every experimental stage, the effectiveness of Analytic Hierarchy Process-Entropy weight, Fuzzy-Rough Set Theory, Analytic Hierarchy Process-Structural Equation Model, and Particle Swarm Optimization-Cuckoo Search optimizing indexes is verified. The results show that: (1) About index optimization models, Particle Swarm Optimization-Cuckoo Search and Analytic Hierarchy Process-Entropy weight are better than Fuzzy-Rough Set Theory, so both Particle Swarm Optimization-Cuckoo Search and Analytic Hierarchy Process-Entropy weight could optimize index predicting overall comfort. (2) Different movements have great differences in the parts that affect the overall comfort.