Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study. - Université de Lille Accéder directement au contenu
Article Dans Une Revue European Journal of Histochemistry Année : 2018

Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study.

Roberta Sferra
  • Fonction : Auteur
Simona Pompili
  • Fonction : Auteur
Luca Ventura
  • Fonction : Auteur
Caroline Dubuquoy
  • Fonction : Auteur
Eugenio Gaudio
  • Fonction : Auteur
Giovanni Latella
  • Fonction : Auteur
Antonella Vetuschi
  • Fonction : Auteur

Résumé

A concomitant action of multiple profibrotic mediators appears crucial in the development and progression of fibrosis. Sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-beta/Smads pathways are both involved in pathogenesis of fibrosis in several organs by controlling differentiation of fibroblasts to myofibroblasts and the epithelial to-mesenchymal transition. However, their direct involvement in chronic colitis-associated fibrosis it is not yet known. In this study we evaluated the immunohistochemical expression of some proteins implicated in sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-beta/Smads pathways in Dextrane Sodium Sulphate (DSS)-induced colorectal fibrosis in mice. Compared to control mice, DSS-induced chronic colitis mice developed a marked intestinal fibrosis associated with a concomitant overexpression of TGF-beta, p-Smad3, alpha-SMA, collagen I-III, SPHK1, RhoA, PI3K, Akt, p-Akt, p-mTOR. This study highlights the relationship between the two pathways and the possible role of SPHK1 in the intestinal fibrosis. These results, if confirmed by in vitro studies, may have important clinical implications in the development of new therapeutical approaches in inflammatory bowel disease.
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Origine : Publication financée par une institution
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

hal-04490158 , version 1 (22-03-2024)

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Paternité - Pas d'utilisation commerciale

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Citer

Roberta Sferra, Simona Pompili, Luca Ventura, Caroline Dubuquoy, Silvia Speca, et al.. Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study.. European Journal of Histochemistry, 2018, European Journal of Histochemistry, 62, ⟨10.4081/ejh.2018.2956⟩. ⟨hal-04490158⟩

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