This study aims to achieve intelligent monitoring of clothing comfort in motion, providing data and technical assistance for the research of comfort in motion and an efficient design foundation for sportswear comfort optimization. In view of the present situation of smart wear used in clothing comfort research, this paper primarily presents the construction of an acquisition system in motion, including temperature, humidity, and pressure data acquisition device, data pre-processing, data storage, and so on, which was called the ATHPD system for short. With the help of this acquisition system, tights in motion may now be tested for pressure, heat, and humidity for the first time. It can also transmit data wirelessly. Meanwhile, in order to verify the effectiveness of the acquisition system, by comparing the measurement data of the acquisition system (ATHPD system) with the measurement data of existing acquisition equipment (AMI3037 pressure measurement system, DS1923 button temperature and humidity recorder). The findings demonstrate that there is no significant difference between the data collected by the two methods, which proves the reliability of the acquisition system in this paper. The system guarantees the same quantity of temperature, humidity, and pressure data to be gathered at the same time and the consistency of the corresponding time points and also provides relevant data support for the evaluation and prediction model construction of human comfort in motion.