In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells. - Université de Lille
Article Dans Une Revue Toxicology in Vitro Année : 2021

In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells.

Résumé

The SH-SY5Y cell line is commonly used for the assessment of neurotoxicity in drug discovery. These neuroblastoma-derived cells can be differentiated into neurons using many methods. The present study has compared 24 of these differentiation methods on SH-SY5Y cells. After morphologic selection of the three most differentiating media (retinoic acid in 10% fetal bovine serum (FBS), staurosporine in 1% FBS medium, and cyclic adenosine monophosphate (cAMP) in B21-supplemented neurobasal medium), cells were analyzed for pan-neuronal and specific neuronal protein expression by fluorescent automated imaging. The response of SH-SY5Y to a set of compounds of known toxicity was examined in these culture conditions performed in 2D, and also in a 3D hyaluronic acid-based hydroscaffold™ which mimics the extracellular matrix. The extent of neuronal markers expression and the sensitivity to neurotoxic compounds varied according to the differentiation medium. The cAMP B21-supplemented neurobasal medium led to the higher neuronal differentiation, and the higher sensitivity to neurotoxic compounds. The culture in 3D modified the neurotoxic response, through a lower sensitivity of cells compared to the 2D culture. The in vitro differentiation environment influences the neurotoxic response of SH-SY5Y cells and thus should be considered carefully in research as well as in drug discovery.
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hal-04769285 , version 1 (13-11-2024)

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Véronique de Conto, V. Cheung, G. Maubon, Z. Souguir, N. Maubon, et al.. In vitro differentiation modifies the neurotoxic response of SH-SY5Y cells.. Toxicology in Vitro, 2021, Toxicology in Vitro, 77, pp.105235. ⟨10.1016/j.tiv.2021.105235⟩. ⟨hal-04769285⟩

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